一.大鼠S100钙结合蛋白A8(S100A8)ELISA试剂盒详细介绍
产品货号:SBJ-R0821
中文名称:大鼠S100钙结合蛋白A8(S100A8)ELISA试剂盒
规格: 96T/48T
品牌:森贝伽
种属:大鼠ELISA试剂盒
检测波长:450 nm
所需样本体积: 50-100ul
适用范围:仅供科研
保存及有效期:2-8℃,六个月
检测目的:用于测定血清,血浆及相关液体等样本。例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
森贝伽供应的种属有:人、大鼠、小鼠、豚鼠、兔子、猪犬、牛羊、鸡鸭、植物ELISA试剂盒等
二.订货信息: 大鼠S100钙结合蛋白A8(S100A8)ELISA试剂盒 货号 | 名称 | 规格 | 品牌 | 检测方法 | 货期 | 价格 |
SBJ-R0821 | 大鼠S100钙结合蛋白A8(S100A8)ELISA试剂盒 | 96T/48T | 森贝伽 | ELISA | 现货 | 点击订购 |
【独特优势】
1、产品种类齐全、质量可靠、价格优惠、灵敏度高、效果稳定、易保存、操作简便
2、免费提供产品最新报价、实验原理、产品用途及中英文说明书
3、发货及时(南京本地客户有专门人员送货上门及取标本)
4、免费提供ELISA代测服务,森贝伽拥有自己的实验室(北京、南京、上海、武汉)和技术团队,公司提供全方位的售前、售中、售后技术支持,为您解决实验过程中遇到的问题。想要了解更多森贝伽实验代做服务,请来电咨询或点击ELISA常见问题页面。
三.森贝伽ELISA试剂盒组成: 产品组成 | 48孔配置 | 96孔配置 | Storage |
标准品:180ng/L | 0.5ml×1瓶 | 0.5ml×1瓶 | 2-8℃ |
标准品稀释液 | 1.5ml×1瓶 | 1.5ml×1瓶 | 2-8℃ |
酶标试剂 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
样品稀释液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
显色剂A液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
显色剂B液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
终止液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃ |
浓缩洗涤液 | (20ml×20倍)×1瓶 | (20ml×30倍)×1瓶 | 2-8℃ |
酶标包被板 | 1×48 | 1×96 | 2-8℃ |
封板膜 | 2片 | 2片 | RT |
密封袋 | 1个 | 1个 | RT |
说明书 | 1份 | 1份 | RT |
四.操作步骤: 五.注意事项:1.试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
2.浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
3.各步加样均应使用加样器,并经常校对其准确性,以避免试验误差。一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排枪加样。
4.请每次测定的同时做标准曲线,最好做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请最后乘以总稀释倍数(×n×5)。
5.封板膜只限一次性使用,以避免交叉污染。
6.底物请避光保存。
7.严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8.所有样品,洗涤液和各种废弃物都应按传染物处理。
9.本试剂不同批号组分不得混用。
10.如与英文说明书有异,以英文说明书为准。
六.标准曲线的绘制方法:
可以采用各种绘图软件来绘制ELISA标准曲线,我公司以“Curve Exert1.3”软件为例,绘制的ELISA标准曲线如下:
计算:以标准物的浓度为横坐标,OD值为纵坐标,在坐标纸上绘出标准曲线,根据样品的OD值由标准曲线查出相应的浓度;再乘以稀释倍数;或用标准物的浓度与OD值计算出标准曲线的直线回归方程式,将样品的OD值代入方程式,计算出样品浓度,再乘以稀释倍数,即为样品的实际浓度
七.特别声明:南京森贝伽长期专业供应各类科研产品,包括ELISA试剂盒、生物试剂、标准品对照品、培养基、抗体、染色液染色试剂盒等优质产品。森贝伽本着客户至上的原则为客户提供高质量高品质的产品,公司承诺如有任何质量的问题请打电话与我们工作人员联系,免费包退换(非人为因素造成的)会给您一个满意的答复。欢迎来电咨询大鼠S100钙结合蛋白A8(S100A8)ELISA试剂盒最新报价、实验原理、产品用途及中英文说明书!
标签:大鼠S100钙结合蛋白A8(S100A8)ELISA试剂盒 大鼠S100钙结合蛋白A8 大鼠ELISA试剂盒
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作者单位:东南大学
使用产品:Human MCP-1, TNF-α, TGF-β ELISA kit
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13、Siyuan Cui,Lili Men,Yu Li,etc.Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells[J].Mediators Of Inflamm.2018:1-16.
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使用产品:Human ICAM-1, VCAM-1 ELISA kit
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作者单位:河北医科大学第二医院
使用产品:Human CCL19, CCL22 ELISA kit
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使用产品:Human cAMP, CEA, SCC ELISA kit
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作者单位:中国医学科学院中国协和医科大学药物研究所
使用产品:Human MDA ELISA kit
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23、Ying Huang,Shaofeng Wang,Chunya Zhang,etc.Experimental study of the anti-atherosclerotic effect of demethylzeylasteral[J].Exp Ther Med.2017,13(6): 2787-2792.
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使用产品:Plant AHL ELISA Kit
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使用产品:Mouse CRP, LPS ELISA Kit